Anti-inflammatory secondary metabolites from a Beihai mangrove endophytic fungus Aspergillus sp. GXIMD00016
The mangrove endophytic fungus Aspergillus sp. GXIMD00016 was fermented by using rice medium. The metabolites were isolated by the chromatography technology and seven compounds were obtained. By analyzing MS and NMR spectroscopic data, their structures were identified as aculeatusquinone A (1), aculeatusquinone C (2), roseopurpurin A (3), myxotrichin C (4), 2, 7-didechlorovicanic (5), atraric acid (6) and 2, 5-dimethyl-1, 3-benzenediol (7). Besides, compound 5 was a new natural product, and compounds 3 and 4 were isolated from Aspergillus sp. for the first time. The inhibitory effects of compounds 1-3 and 5-7 on lipopolysaccharide (LPS) induced NO production were evaluated in mouse macrophage RAW264. 7 cells. The results showed that compounds 1-3 had a significant anti-inflammatory activity with IC50 values ranging from 16. 68 to 28. 15 μmol/L.